Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3

公開日:2012.10.01

発行日
2002年03月31日
概要
The whole caaF-caaE-caaABCD gene cluster, contains two open reading frames (caaE and caaF seem to be coding for the proteins related to heme biosynthesis pathway) on the 5’-upstream of the caa3-type cytochrome
c oxidase structural genes (caaA, caaB, caaC, and caaD), was isolated from a genomic DNA library of the thermophilic bacterium Bacillus PS3. The caaE gene and its flanking regions were identified. The deduced amino acid sequence of the caaE product is composed of 309 amino acid residues, and is homologous to that
of the cyoE product (heme O synthase) of bo-type ubiquinol oxidase operon from Escherichia coli. The caaE is located on the 5’-upstream of the caaA (corresponds to subunit II of PS3 cytochrome c oxidase) apart from 77 base pairs, and there are a large stem-loop structure and promoter-like structures in this region. This promoter-like structure was needed for the in vivo expression of caaA from PS3 in E. coli. The caaF is located on the 5’-upstream of the caaE apart from 175 base pairs in the reverse direction. A recombinant E. coli cell harboring the caaF-caaE-caaABCD gene cluster of PS3 synthesized heme A. The product of caaE (CaaE) was expressed in E. coli, and had a thermostable heme O synthase (protoheme IX farnesyltransferase) activity in cytoplasmic membrane [Saiki, K., Mogi, T., Ishizuka, M., and Anraku, Y., FEBS Lett. 351(2), 385-388, 1994]. Furthermore, PS3 CaaE amino acid residues as candidates for taking part in prenyl binding, transfer, or structure preserving, and for participating heme B binding or structure preserving by the comparison among CaaE homologues are discussed.【査読有】
文献等

掲載誌名・書名:

中央大学理工学研究所論文集, 第7号/2001, pp.11-28

公開者・出版社:

中央大学理工学研究所

書誌コード類:

ISSN: 1343-0068

種類
論文
言語
英語
権利情報
この資料の著作権は、資料の著作者または学校法人中央大学に帰属します。著作権法が定める私的利用・引用を超える使用を希望される場合には、掲載誌発行部局へお問い合わせください。

このページのTOPへ